screening of anticancer plant extracts using culture

In Vitro and In Vivo Anticancer Activity of Root Extracts ...

Introduction. Sansevieria liberica Gerome and Labroy (Agavaceae) is a perennial plant widely distributed in tropical Africa. Preparations of the plant are commonly used across Nigeria for the treatment of inflammatory conditions. Based on the fact that herbal medicine is a strong component of integrative medicine, this study was conducted to evaluate the anticancer activity of root extracts of ...

In-vitro Anti-Cancer Activity of Extracts Dracaen ...

unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract Plants have a long history of use in treatment of cancer, Since many years, plants were known to possess anticancer activities against different cancer cell lines.

295 High throughput screening of potential anti-cancer ...

This method allows HTS of compound collections for anticancer activity in primary cell cultures. In a pilot study [51], 5605 plant extracts were each screened against primary cultures established ...

In vitro anticancer activity and cytotoxicity screening of ...

extract (EAE), n-butanol extract (BE), the remainder after extraction (RE) and water extract (WE) from each plant were prepared. These 60 extracts were screened for their cytotoxicity on the aforementioned cell lines using MTT assay. Results: All plant species showed certain anticancer activi-

EVALUATION OF ANTIMICROBIAL ACTIVITY OF DIFFERENT …

The different herbal plant extracts are traditionally has been used as anticancer antioxidant, antiulcer, analgesic and antidiabetic 12, and they also having the antiparasitic, antifungal, antibacterial, antimalarial activity, analgesic and anti-inflammatory activity 13. The below mentioned plants were used to study the antimicrobial activity.

Silver Nanoparticle Synthesis from Plant Extracts and its ...

plant extracts have been extensively used [4]. 2. REVIEW OF THE LITERATURE Deshpande Raghunandhan et al., (2011) [5] synthesized gold & silver nanoparticles using an aqueous extract of guava leaves and clove buds. The experimental procedure was to found out the invitro anticancer activity against 4 human cell lines.

Which method is most suitable and accurate for …

Which method is most suitable and accurate for screening anticancer activity of plant extract? We want to screen pant extracts for anticancer activity by using cell culture or cell line ...

SCREENING OF SOLVENT EXTRACT OF PSIDIUM …

│ Vol 8, Issue 5, 2021.│ ISO 9001:2015 Certified Journal │ Anusree et al. European Journal of Pharmaceutical and Medical Research 417 SCREENING OF SOLVENT EXTRACT OF PSIDIUM CATTLEIANUM LEAVES FOR ANTICANCER ACTIVITY AGAINST HEPATIC CANCER CELL LINE Anusree K. V.1* and Dhivya R.2 1Student, PG and Research Department of Zoology, Nirmala …

Phytochemical screening and anticancer activity of the ...

Cancer is one of the most considerable concerns because of increasing the death rate all over the world. Recent studies have disclosed that plant extracts exhibit anticancer activity through various mechanisms. Xanthium strumarium has been used by Vietnamese in herbal medicines to support the medication of infirmities. This study is to consider the secondary metabolites, antioxidant and ...

Screening of antimicrobial activity of Withania somnifera ...

The pathogenic microorganisms are sub cultured 3 times at 37 0C for 24 h before the usage and 1ml of culture inoculated into nutrient broth and incubated at 37 0C for 24 h. Overnight cultures were used to test the antibacterial activity of plant extracts by using well diffusion method Table 1.

In vitro anti-cancer efficacy and phyto-chemical screening ...

Laboratory data on its anti-cancer activity and active principles is limited, hence no scientific rationale for its medicinal use. This study reports on in-vitro toxic activities of dichloromethane and methanol extracts of the plant against human breast cancer cells and phytochemical screening of the two extracts.

U.S. GAO - Review of the National Cancer Institute's ...

A review of the DTP research program was undertaken which focused on the activities within two branches: (1) the Natural Products Branch (NPB), which arranges for acquiring plants; and (2) the Drug Evaluation Branch (DEB), which is responsible for screening new agents for anticancer activity. NPB arranges for worldwide collection of plants.

In Vitro Screening for Cytotoxic Activity of Herbal Extracts

3. Results. To demonstrate the potential biological activity of alcoholic extracts, we carried out a screening of the extracts by measuring their in vitro antiproliferative properties against several common human cancer cell lines. Some plant extracts exhibited …

ANTICANCER, ANTIBACTERIAL, AND ANTIFUNGAL …

Many plants and medicinal herbal extracts are used in the treatment of various ailments. Herbal medicine (botanical medicine or phytomedicine) is a concept used to describe the usage of plant's roots, seeds, bark, stems, flowers, or leaves for medicinal purposes.

Research Article Bauhinia variegata Leaf Extracts Exhibit ...

bacterial culture was maintained at C on nutrient agar slants.. . Evaluation of Antimicrobial Activity. Antimicrobial activity of plant extracts was determined using Kirby- disc di usion method [ ]. e inoculum suspension of bacterial strains was swabbed on the entire surface of Mueller-Hinton agar (MHA). Sterile mm diameter paper

Screening of Antioxidants from Medicinal Plants for ...

Abstract: Doxorubicin is an important and effective anticancer drug widely used for the treatment of various types of cancer but its clinical use is limited by dose-dependent cardiotoxicity. Elevated tissue levels of cellular superoxide anion/oxidative stress are a mechanism by which doxorubicin-induced cardiotoxicity. Selected medicinal plant extracts were tested for their antioxidant ...

In vitro anticancer activity and cytotoxicity screening of ...

Methods: Six phytochemical extracts, namely petroleum ether extract (PEE), chloroform extract (CE), ethyl acetate extract (EAE), n-butanol extract (BE), the remainder after extraction (RE) and water extract (WE) from each plant were prepared. These 60 extracts were screened for their cytotoxicity on the aforementioned cell lines using MTT assay.

Screening for selective anticancer activity of plants from ...

Since plants are an important source of anticancer drugs, we have carried out a random screening for selective anticancer activity of 57 extracts from 45 plants collected in Grazalema Natural Park, an area in the South of Spain of high plant diversity and endemism. Using lung cancer cells (A549) and …

In vitro anticancer activity of ethanol extract of ...

Crude extracts. The extracts of the A. vasica plant were done using the cold percolation method using 70% ethanol as solvent and were kept in a shaker for 48–72 h. Afterwards, they were squeezed using a muslin cloth and the extract was kept for drying. The dried extract was used for the analysis in the whole study.

Anticancer potential of selected Fallopia Adans species

potential of these compounds, a number of plant extracts rich in flavonoids have been investigated in order to evaluate their anti-cancer properties (19,20). The low production cost of the plant extracts compared to the pure compounds and the synergistic effects of the natural compounds are the main advantages for using natural extracts (21).

Development of a High-Throughput Screening System to ...

identify over 88,000 extracts with anticancer properties. However, the use of whole plants to generate these specialized metabolites has numerous drawbacks, specifically the low and inconsistent production of compounds. This project aims to improve upon this screening system and develop a high-throughput screening system with the use of plant ...

Discovery of novel inducers of cellular differentiation ...

Non-physiological inducers of terminal differentiation have been used as novel therapies for the prevention and therapy of cancer. We have used cultured HL-60 promyelocytic cells to monitor differentiation, proliferation and cell death events as induced by a large set of extracts derived from plants. Screening of more than 1400 extracts led to ...

(PDF) The study of anticancer and antifungal activities of ...

The stem bark activity and qualitative phytochemical analysis of the extract of Taxus brevifolia (Taxaceae) and different parts plant extract have also been demonstrated. of Camptotheca acuminata (Nyssaceae) also proved active during anticancer screening and finally lead to the The fresh P. integerrima Stew. ex Brand plant material isolation of ...

Thieme E-Journals - Planta Medica / Full Text

Table 1 Cell-based assays in use for screening for anticancer activities. Target. Formats in use ..., 5605 plant extracts were each screened against primary cultures established from three recurrent ovarian tumours. Sixty extracts were active, and 13 of these had previously been found to be positive in a breast cancer cell line screen (ZR75 ...

Screening of Plants Used as Traditional Anticancer ...

Screening of Plants Used as Traditional Anticancer Remedies in Mkuranga and Same Districts, Tanzania, Using Brine Shrimp Toxicity BioassayAuthors: Daniel Z. Matata, OlipaNgassapa, Francis Machumi and Mainen J. Moshi.Presenter: Dr. Pax J. MasimbaContact: Institute of Traditional Medicine, Muhumbili University of Health and Allied Sciences, Dar es Salaam, Tanzania: email: …

In-vitro antioxidant, anti-cancer, and anti-inflammatory ...

Keywords: Medicinal plants, Anticancer, Anti-inflammatory, Antioxidant Background The use of plant-based medicines for the treatment of various ailments is practiced from ancient times such as Ayurveda, Unani, and Siddha, and has been part of hu-man culture [1–3]. The knowledge of chemical constitu-ents of plants is desirable for the discovery of

In vitro Anticancer Screening of 24 Locally Used Nigerian ...

In vitro anticancer screening of 24 locally used Nigerian medicinal plants Saudat Adamson Fadeyi1,3, Olugbeminiyi O Fadeyi2,4, Adedeji A Adejumo5, Cosmas Okoro2 and Elbert Lewis Myles1* Abstract Background: Plants that are used as traditional medicine represent a relevant pool for selecting plant candidates that may have anticancer properties.

Correlation between cytotoxicity in cancer cells and free ...

Rules-based analysis using Matthew's correlation coefficient (MCC) scores and enrichment factors as criteria for the evaluation of the models' efficiency revealed that the plant extracts whose EC 50 for free radical scavenging ≤10 µg/ml showed some degree of enrichment toward more cytotoxicity ().The values for the enrichment factor, the MCC, accuracy, and precision are 2.6, 0.28, …