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The PCR conditions consisted of 2 min of initial denaturation at 95 °C, 40 cycles of denaturation at 95 °C for 5 s and annealing at 60 °C for 30 s, and lastly, 1 min of activation at 95 °C, annealing at 55 °C for 15 s and elongation at 95 °C for 30 s. Each experiment was performed in triplicate. In vivo assays Biodistribution

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Cells with primary cilia were rinsed in the planchets with PBS for 10 min at 37 °C, and then fixed with 2.5% glutaraldehyde in 37 °C PBS buffer for 0.5 h, and at room temperature for 0.5 h. The cells in planchets were rinsed with PBS once for 10 min followed by soaking in 0.05% poly-lysine (Sigma) solution in PBS for 3 min at room temperature.

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Incubation was performed at 37°C for 2 h 30 min with agitation. The gel was washed 5 × 10 min with 2 ml 0.5% Tween-20/PBS afterward. For NHS-ester staining, the samples were additionally incubated with Atto594-NHS-Ester (ATTO-TEC, AD 594-31) at 10 μg/ml in PBS shaking for 1 h 30 min at RT and washed 3 × 15 min with 0.1% Tween-20/PBS.

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